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KMID : 1161520010050030205
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Animal Cells and Systems
2001 Volume.5 No. 3 p.205 ~ p.209
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Inhibition of chondrogenes is by cytochalasin D in high density micromass culture of chick mesenchymal cells: Its effects on expression of a-smooth muscle Actin and P-cadhe rin
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Yoo Jeong-Ah
Park Su-Jung
Kang Shin-Sung
Park Tae-Kyu
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Abstract
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Mesenchymal cells from the leg buds of stage 24?chick embryos differentiated into chondrocytes when plated at high density. Treatment of high density micromass culture of chick mesenchymal cells with cytochalasin D (CD, 2 μM for 24 h) resulted in inhibition of chondro gene sis. CD treatment was found to affect the expression of the contractile protein a?smooth muscle actin (a?SM actin). In control cultures, a?SM actin uniformly expressed from culture day 2, but the CD?treated cells induced expression of a?SM actin from the first day of culture followed by a continuous increase. Expression of pan?cadherin (P?cadherin) decreased as chondro gene sis proceeded in the control culture, whereas the CD?treated cells showed sustained expression. These results propose a close connection of chondrogenic differentiation with expression of a?SM actin and P?cadherin.
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KEYWORD
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Chondrogenesis, Cytochalasin D, Actin cytoskeleton, α-Smooth muscle actin, pan-cadherin
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